The Red Mullions Guest House in Headington, Oxford, has been placed on the market for £2.5 million after nearly 20 years of ownership and operation by the current proprietors.
The owners said they intend to retire and are seeking buyers who will continue to operate the family-run business.
Fleurets is handling the sale.
The estate agents described the property as being in excellent condition and said a purchaser would be able to continue trading without disruption.
The owners also thanked the guest house’s staff and customers, some of whom have become friends.
They hope eventually to transfer the business to new owners who will share their enthusiasm for it.
Trading is continuing in the meantime.
The hotel has 16 letting rooms, including 15 en-suite rooms.
Seven are on the ground floor, six on the first floor and three on the second floor.
The property also includes a restaurant with 22 covers, kitchen facilities, parking spaces and a garden at the rear.
The listing describes Red Mullions as a substantial detached building constructed mainly across the ground and first floors.
Its rendered and brick elevations are covered by a pitched tile roof, while a single-storey building at the rear contains private and staff accommodation.
The premises are said to be in excellent condition, allowing a new owner to purchase the business and carry on trading.
The four-star guest house is described as a popular destination among tourists.
It holds a 4.6 rating from more than 200 Google Reviews, with reviewers frequently praising the welcoming staff.
One visitor described it as a fantastic bed and breakfast, particularly suitable for people attending a Brooks University open day, adding that the rooms were immaculately clean and that the service was good.
Other reviews highlighted the food served at the guest house.
A free continental breakfast is provided with every booking made directly with Red Mullions.