A planning application seeking permission for 130 homes on fields west of Chalgrove Airfield has attracted strong opposition from residents in the South Oxfordshire village.
The proposed site lies beside land approved for 200 homes in 2022 near the B480.
Chalgrove currently has approximately 1,200 homes and 3,000 residents, having expanded rapidly during the 1960s.
Previous Homes England plans could result in 3,400 additional homes in the village.
Residents have raised concerns about the possible effect of the latest scheme on the surrounding countryside.
Maria do Carmo said the area provides space for walking and is regularly visited by deer, hares and other wildlife.
She opposed construction on the site and called for the animals to retain a habitat.
The outline application includes 130 dwellings, 40 per cent of which would be affordable, together with a play area and substantial public open space and landscaping.
Ricky Allen, who lives close to the proposed development, said building 130 homes could cause lasting harm to Chalgrove and alter the character of the surrounding landscape by extending the built environment into the countryside.
He asked the council to examine the consequences for rural views, the established pattern of settlement and the relationship between the village and its surrounding countryside.
Mandy Hough also raised concerns about the infrastructure required to support further housing growth.
She said Chalgrove already has a growing number of homes but insufficient shops, roads, doctor’s appointments and school places.
She believes the village should not accommodate additional development at the expense of its existing character and amenities.